The Fluorescent Staining of Bacteriophage Nucleic Acids
نویسندگان
چکیده
منابع مشابه
Fluorescent Nucleic Acids
A new modular approach to the design and discovery of fluorophores and sensors built on a DNA backbone is described. Fluorescent designer nucleosides are synthesized into short oligomers; their close interactions results in highly efficient reporters with properties not seen in standard fluorophores. Applications in sensing of molecules, ions, and enzymes in water and in the vapor phase are des...
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Borrelia burgdorferi the Lyme disease spirochete was grown in modified Kelly medium and characterized by transmission and by scanning electron microscopy. Using silver staining procedures which preferentially bind to nuclear components of eukaryotic cells, signal could be detected by backscattered electron imaging throughout the length of the prokaryotic spirochete. Interestingly, however, the ...
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Silver staining was introduced more than a decade ago as a sensitive procedure to detect trace amounts of proteins in polyacrylamide gels (1). Since then, silver staining has been perfected and extended to the study of other biological molecules that have been separated by electrophoresis in a variety of supports (2,3). Silver staining follows generally one of two methods. One uses diamine or a...
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Nucleic acids are typically detected in morphologically preserved fixed cells and tissues using in situ hybridization techniques. This review discusses a variety of established and more challenging fluorescence-based methods for the detection and tracking of DNA or RNA sequences in living cells. Over the past few years, various fluorescent in vivo labeling methods have been developed, and dedic...
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SYNTHESIS 2006, No. 4, pp 0645–0653xx.xx.2006 Advanced online publication: 25.01.2006 DOI: 10.1055/s-2006-926313; Art ID: M05505SS © Georg Thieme Verlag Stuttgart · New York Abstract: Starting from glycidol, the synthesis of dimethoxytritylated glycol nucleoside phosphoramidites of adenine (A), thymine (T), uracil (U), guanine (G), and cytosine (C) is reported. These phosphoramidites are the bu...
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ژورنال
عنوان ژورنال: Journal of General Microbiology
سال: 1966
ISSN: 0022-1287
DOI: 10.1099/00221287-44-3-383